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1x tae buffer  (Bio-Rad)


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    Bio-Rad 1x tae buffer
    1x Tae Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+tae+buffer/EDTA/pm41774180-92-10-47
    Average 96 stars, based on 1389 article reviews
    1x tae buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Characterisation of lactic acid bacteria and yeast strains isolated from traditional yoghurts to obtain new starter culture combinations and the properties of products during the storage period
    Article Snippet: Taq DNA polymerase (Fermentas) was used at 7.5 and 2.5 U for lactobacilli and enterococci and lactococci, respectively. .. PCR was performed in a thermal cycler (Bio-Rad T100, Hercules, CA, USA) as follows: initial denaturation at 95 ◦C for 5 min, followed by 35 cycles of 95 ◦C for 45 s, 42.4 ◦C for 90 s, and 72 ◦C for 45 s. PCR products were prepared according to the expected amplicon size in 1%– 2% agarose (Sigma) gel in 1X TAE buffer (0.04 M Tris-acetate, 0.001 M EDTA) and run in a horizontal electrophoresis system (Bio-Rad) at 90 V for 40 min. A 100 bp DNA ladder (Fermentas, SM0242) and a 1 kb DNA ladder (Fermentas, SM0311) were used as markers. .. The gel stained with GelRed nucleic acid gel stain (Biotium, Inc., Fremont, CA, USA) was visualised using an imaging system (Bio-Rad, Gel Doc Ez Imager).

    Article Title: Microcystis aeruginosa detected in freshwater lakes in Meghalaya, India show strong hepatotoxicity.
    Article Snippet: microcystin producers such as Microcystis aeruginosa as one of the prominent agents of hepatotoxicity (Singh et al. 2015).. The high hepatotoxicity of microcystin has promoted research in developing MC-biosensor (Antonyraj et al. 2025).. M. aeruginosa is a ubiquitous freshwater cyanobacterium known for its harmful impact on aquatic and terrestrial organisms through toxic bloom formation and the release of the hepatotoxic microcystin.

    Article Title: Development of SolyCEL3 marker to evaluate graft incompatibility and growth performance of tomato (Solanum lycopersicum) scions on tomato and eggplant (Solanum melongena) rootstocks
    Article Snippet: Conventional polymerase chain reactions (PCR) containing 20 ng of genomic DNA template, 1.25 units of TaKaRa Ex Taq® DNA Polymerase (TaKaRa Bio Cat. No. RR001B), 200 mM of dNTP, 200 ng of primer, and PCR buffer at a final concentration of 2 mM MgCl2 were performed in a final volume of 50 ml following manufacturer instructions. .. The PCR was performed in an ABI GeneAmp PCR System 9700 (Applied Biosystems, USA) set with the following thermal program: initial denaturation at 98 °C for 30 s, followed by 30 cycles of 98 °C for 10 s, 61 °C (F1/R1) and 55 °C (F2/R2) for 30 s, and 72 °C for 2 min, with a final extension at 72 °C for 10 min. Amplified products were separated by electrophoresis on 1% agarose gel in a 1x TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with ethidium bromide and made visible by UV light using Molecular Imager® Gel DocTM XR+ Imaging System (Bio-Rad). ..

    Article Title: Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, and H. pylori .
    Article Snippet: .. The PCR products were analyzed by agarose gel electrophoresis using 2 % agarose gel in 1X TAE buffer (Tris-Acetic-EDTA), stained with 0.02 % SYBR® Safe–DNA gel stain (Invitrogen, USA); electrophoresis was carried out at 80 V for 60 min. After completion, the presence of amplified fragments was visualized using the Gel DocTM XR + Imaging System Molecular Imager (BIO-RAD, USA). ..

    Amplification:

    Article Title: Characterisation of lactic acid bacteria and yeast strains isolated from traditional yoghurts to obtain new starter culture combinations and the properties of products during the storage period
    Article Snippet: Taq DNA polymerase (Fermentas) was used at 7.5 and 2.5 U for lactobacilli and enterococci and lactococci, respectively. .. PCR was performed in a thermal cycler (Bio-Rad T100, Hercules, CA, USA) as follows: initial denaturation at 95 ◦C for 5 min, followed by 35 cycles of 95 ◦C for 45 s, 42.4 ◦C for 90 s, and 72 ◦C for 45 s. PCR products were prepared according to the expected amplicon size in 1%– 2% agarose (Sigma) gel in 1X TAE buffer (0.04 M Tris-acetate, 0.001 M EDTA) and run in a horizontal electrophoresis system (Bio-Rad) at 90 V for 40 min. A 100 bp DNA ladder (Fermentas, SM0242) and a 1 kb DNA ladder (Fermentas, SM0311) were used as markers. .. The gel stained with GelRed nucleic acid gel stain (Biotium, Inc., Fremont, CA, USA) was visualised using an imaging system (Bio-Rad, Gel Doc Ez Imager).

    Article Title: Development of SolyCEL3 marker to evaluate graft incompatibility and growth performance of tomato (Solanum lycopersicum) scions on tomato and eggplant (Solanum melongena) rootstocks
    Article Snippet: Conventional polymerase chain reactions (PCR) containing 20 ng of genomic DNA template, 1.25 units of TaKaRa Ex Taq® DNA Polymerase (TaKaRa Bio Cat. No. RR001B), 200 mM of dNTP, 200 ng of primer, and PCR buffer at a final concentration of 2 mM MgCl2 were performed in a final volume of 50 ml following manufacturer instructions. .. The PCR was performed in an ABI GeneAmp PCR System 9700 (Applied Biosystems, USA) set with the following thermal program: initial denaturation at 98 °C for 30 s, followed by 30 cycles of 98 °C for 10 s, 61 °C (F1/R1) and 55 °C (F2/R2) for 30 s, and 72 °C for 2 min, with a final extension at 72 °C for 10 min. Amplified products were separated by electrophoresis on 1% agarose gel in a 1x TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with ethidium bromide and made visible by UV light using Molecular Imager® Gel DocTM XR+ Imaging System (Bio-Rad). ..

    Article Title: Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, and H. pylori .
    Article Snippet: .. The PCR products were analyzed by agarose gel electrophoresis using 2 % agarose gel in 1X TAE buffer (Tris-Acetic-EDTA), stained with 0.02 % SYBR® Safe–DNA gel stain (Invitrogen, USA); electrophoresis was carried out at 80 V for 60 min. After completion, the presence of amplified fragments was visualized using the Gel DocTM XR + Imaging System Molecular Imager (BIO-RAD, USA). ..

    Electrophoresis:

    Article Title: Characterisation of lactic acid bacteria and yeast strains isolated from traditional yoghurts to obtain new starter culture combinations and the properties of products during the storage period
    Article Snippet: Taq DNA polymerase (Fermentas) was used at 7.5 and 2.5 U for lactobacilli and enterococci and lactococci, respectively. .. PCR was performed in a thermal cycler (Bio-Rad T100, Hercules, CA, USA) as follows: initial denaturation at 95 ◦C for 5 min, followed by 35 cycles of 95 ◦C for 45 s, 42.4 ◦C for 90 s, and 72 ◦C for 45 s. PCR products were prepared according to the expected amplicon size in 1%– 2% agarose (Sigma) gel in 1X TAE buffer (0.04 M Tris-acetate, 0.001 M EDTA) and run in a horizontal electrophoresis system (Bio-Rad) at 90 V for 40 min. A 100 bp DNA ladder (Fermentas, SM0242) and a 1 kb DNA ladder (Fermentas, SM0311) were used as markers. .. The gel stained with GelRed nucleic acid gel stain (Biotium, Inc., Fremont, CA, USA) was visualised using an imaging system (Bio-Rad, Gel Doc Ez Imager).

    Article Title: Development of SolyCEL3 marker to evaluate graft incompatibility and growth performance of tomato (Solanum lycopersicum) scions on tomato and eggplant (Solanum melongena) rootstocks
    Article Snippet: Conventional polymerase chain reactions (PCR) containing 20 ng of genomic DNA template, 1.25 units of TaKaRa Ex Taq® DNA Polymerase (TaKaRa Bio Cat. No. RR001B), 200 mM of dNTP, 200 ng of primer, and PCR buffer at a final concentration of 2 mM MgCl2 were performed in a final volume of 50 ml following manufacturer instructions. .. The PCR was performed in an ABI GeneAmp PCR System 9700 (Applied Biosystems, USA) set with the following thermal program: initial denaturation at 98 °C for 30 s, followed by 30 cycles of 98 °C for 10 s, 61 °C (F1/R1) and 55 °C (F2/R2) for 30 s, and 72 °C for 2 min, with a final extension at 72 °C for 10 min. Amplified products were separated by electrophoresis on 1% agarose gel in a 1x TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with ethidium bromide and made visible by UV light using Molecular Imager® Gel DocTM XR+ Imaging System (Bio-Rad). ..

    Article Title: Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, and H. pylori .
    Article Snippet: .. The PCR products were analyzed by agarose gel electrophoresis using 2 % agarose gel in 1X TAE buffer (Tris-Acetic-EDTA), stained with 0.02 % SYBR® Safe–DNA gel stain (Invitrogen, USA); electrophoresis was carried out at 80 V for 60 min. After completion, the presence of amplified fragments was visualized using the Gel DocTM XR + Imaging System Molecular Imager (BIO-RAD, USA). ..

    Size Selection:

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci.
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102 ), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Staining:

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci.
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102 ), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Article Title: Microcystis aeruginosa detected in freshwater lakes in Meghalaya, India show strong hepatotoxicity.
    Article Snippet: microcystin producers such as Microcystis aeruginosa as one of the prominent agents of hepatotoxicity (Singh et al. 2015).. The high hepatotoxicity of microcystin has promoted research in developing MC-biosensor (Antonyraj et al. 2025).. M. aeruginosa is a ubiquitous freshwater cyanobacterium known for its harmful impact on aquatic and terrestrial organisms through toxic bloom formation and the release of the hepatotoxic microcystin.

    Article Title: Development of SolyCEL3 marker to evaluate graft incompatibility and growth performance of tomato (Solanum lycopersicum) scions on tomato and eggplant (Solanum melongena) rootstocks
    Article Snippet: Conventional polymerase chain reactions (PCR) containing 20 ng of genomic DNA template, 1.25 units of TaKaRa Ex Taq® DNA Polymerase (TaKaRa Bio Cat. No. RR001B), 200 mM of dNTP, 200 ng of primer, and PCR buffer at a final concentration of 2 mM MgCl2 were performed in a final volume of 50 ml following manufacturer instructions. .. The PCR was performed in an ABI GeneAmp PCR System 9700 (Applied Biosystems, USA) set with the following thermal program: initial denaturation at 98 °C for 30 s, followed by 30 cycles of 98 °C for 10 s, 61 °C (F1/R1) and 55 °C (F2/R2) for 30 s, and 72 °C for 2 min, with a final extension at 72 °C for 10 min. Amplified products were separated by electrophoresis on 1% agarose gel in a 1x TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with ethidium bromide and made visible by UV light using Molecular Imager® Gel DocTM XR+ Imaging System (Bio-Rad). ..

    Article Title: Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, and H. pylori .
    Article Snippet: .. The PCR products were analyzed by agarose gel electrophoresis using 2 % agarose gel in 1X TAE buffer (Tris-Acetic-EDTA), stained with 0.02 % SYBR® Safe–DNA gel stain (Invitrogen, USA); electrophoresis was carried out at 80 V for 60 min. After completion, the presence of amplified fragments was visualized using the Gel DocTM XR + Imaging System Molecular Imager (BIO-RAD, USA). ..

    Molecular Weight:

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci.
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102 ), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Sterility:

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci.
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Article Title: Anthracyclines induce global changes in cardiomyocyte chromatin accessibility that overlap with cardiovascular disease loci
    Article Snippet: .. For gel-based size selection, DNA was loaded onto 2% Ultra Agarose gels (1613107, Bio-Rad) prepared in 1x TAE buffer (1610743, Bio-Rad) containing SYBR Safe DNA stain (Invitrogen, S33102 ), and run alongside a Low Molecular Weight DNA ladder (N3233L, NEB) at 120 V for 40 min. DNA fragments ranging from 250 - 400 bp were visualized using a blue light transilluminator and excised using sterile scalpels. .. Excised gel slices were purified using the MinElute Gel Extraction Kit (28606, Qiagen), following the manufacturer’s protocol.

    Agarose Gel Electrophoresis:

    Article Title: Ancient co-option of LTR retrotransposons as yeast centromeres
    Article Snippet: Chromosomes from S. cerevisiae (0.225–2.2 Mb, Bio-Rad; 1703605) and W. canadensis (1.05–3.13 Mb, Bio-Rad; 170-3667) were used as molecular weight standards. .. Chromosomes were then separated on a 0.8% agarose gel in 1x TAE buffer (Certified Megabase Agarose Bio-Rad, 1613108) following the program details for Hansenula wingei chromosomes, on a BioRad CHEF-mapper XA system. ..

    Article Title: Microcystis aeruginosa detected in freshwater lakes in Meghalaya, India show strong hepatotoxicity.
    Article Snippet: microcystin producers such as Microcystis aeruginosa as one of the prominent agents of hepatotoxicity (Singh et al. 2015).. The high hepatotoxicity of microcystin has promoted research in developing MC-biosensor (Antonyraj et al. 2025).. M. aeruginosa is a ubiquitous freshwater cyanobacterium known for its harmful impact on aquatic and terrestrial organisms through toxic bloom formation and the release of the hepatotoxic microcystin.

    Article Title: Development of SolyCEL3 marker to evaluate graft incompatibility and growth performance of tomato (Solanum lycopersicum) scions on tomato and eggplant (Solanum melongena) rootstocks
    Article Snippet: Conventional polymerase chain reactions (PCR) containing 20 ng of genomic DNA template, 1.25 units of TaKaRa Ex Taq® DNA Polymerase (TaKaRa Bio Cat. No. RR001B), 200 mM of dNTP, 200 ng of primer, and PCR buffer at a final concentration of 2 mM MgCl2 were performed in a final volume of 50 ml following manufacturer instructions. .. The PCR was performed in an ABI GeneAmp PCR System 9700 (Applied Biosystems, USA) set with the following thermal program: initial denaturation at 98 °C for 30 s, followed by 30 cycles of 98 °C for 10 s, 61 °C (F1/R1) and 55 °C (F2/R2) for 30 s, and 72 °C for 2 min, with a final extension at 72 °C for 10 min. Amplified products were separated by electrophoresis on 1% agarose gel in a 1x TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with ethidium bromide and made visible by UV light using Molecular Imager® Gel DocTM XR+ Imaging System (Bio-Rad). ..

    Article Title: Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, and H. pylori .
    Article Snippet: .. The PCR products were analyzed by agarose gel electrophoresis using 2 % agarose gel in 1X TAE buffer (Tris-Acetic-EDTA), stained with 0.02 % SYBR® Safe–DNA gel stain (Invitrogen, USA); electrophoresis was carried out at 80 V for 60 min. After completion, the presence of amplified fragments was visualized using the Gel DocTM XR + Imaging System Molecular Imager (BIO-RAD, USA). ..

    Article Title: Low Density Polyethylene (LDPE) biodegradation efficiency of Bacillus pacificus SBAA07 isolated from Sundarbans mangrove sediments
    Article Snippet: Low-Density Polyethylene (LDPE) contamination in the environment poses a significant ecological threat and harm to human health, prompting extensive research into effective remediation strategies using both chemical and biological approaches.. Among these, bioremediation using bacterial species isolated from diverse habitats has shown considerable promise.. Recent studies indicate that the microbiota of mangrove ecosystems, shaped by extreme and stressed environmental conditions, may exhibit enhanced plastic-degrading capabilities.

    Hood:

    Article Title: Microcystis aeruginosa detected in freshwater lakes in Meghalaya, India show strong hepatotoxicity.
    Article Snippet: microcystin producers such as Microcystis aeruginosa as one of the prominent agents of hepatotoxicity (Singh et al. 2015).. The high hepatotoxicity of microcystin has promoted research in developing MC-biosensor (Antonyraj et al. 2025).. M. aeruginosa is a ubiquitous freshwater cyanobacterium known for its harmful impact on aquatic and terrestrial organisms through toxic bloom formation and the release of the hepatotoxic microcystin.

    Imaging:

    Article Title: Development of SolyCEL3 marker to evaluate graft incompatibility and growth performance of tomato (Solanum lycopersicum) scions on tomato and eggplant (Solanum melongena) rootstocks
    Article Snippet: Conventional polymerase chain reactions (PCR) containing 20 ng of genomic DNA template, 1.25 units of TaKaRa Ex Taq® DNA Polymerase (TaKaRa Bio Cat. No. RR001B), 200 mM of dNTP, 200 ng of primer, and PCR buffer at a final concentration of 2 mM MgCl2 were performed in a final volume of 50 ml following manufacturer instructions. .. The PCR was performed in an ABI GeneAmp PCR System 9700 (Applied Biosystems, USA) set with the following thermal program: initial denaturation at 98 °C for 30 s, followed by 30 cycles of 98 °C for 10 s, 61 °C (F1/R1) and 55 °C (F2/R2) for 30 s, and 72 °C for 2 min, with a final extension at 72 °C for 10 min. Amplified products were separated by electrophoresis on 1% agarose gel in a 1x TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) stained with ethidium bromide and made visible by UV light using Molecular Imager® Gel DocTM XR+ Imaging System (Bio-Rad). ..

    Article Title: Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, and H. pylori .
    Article Snippet: .. The PCR products were analyzed by agarose gel electrophoresis using 2 % agarose gel in 1X TAE buffer (Tris-Acetic-EDTA), stained with 0.02 % SYBR® Safe–DNA gel stain (Invitrogen, USA); electrophoresis was carried out at 80 V for 60 min. After completion, the presence of amplified fragments was visualized using the Gel DocTM XR + Imaging System Molecular Imager (BIO-RAD, USA). ..



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